Elution terms, strategies (stepwise, gradient etc) & practical tips, esp. for protein chromatography
17:17
YouTubethe bumbling biochemist
Elution terms, strategies (stepwise, gradient etc) & practical tips, esp. for protein chromatography
“Elution” is basically just a fancy word for “un-bind” or “come off.” You frequently see it in the context of chromatography as well as various solid phase extraction methods like silica-based micro spin columns for nucleic acid purifications (such as PCR clean up kits). What these methods have in column is that you get a molecule ...
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