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5:57
YouTube
YouTooBio
What is Fluorescence Recovery After Photobleaching (FRAP)?
FRAP is a microscopy technique that scientists use to determine the mobility of proteins inside living cells. In this video, Jayme Dyer explains how FRAP works, and how scientists used FRAP to better understand how cells crawl! The paper discussed in this video is: D. Raz-Ben Aroush, et al., Actin Turnover in Lamellipodial Fragments. Current ...
20.6K views
Nov 2, 2021
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#Fluorescence recovery after photobleaching FRAP Applications
SOLVED: The lateral diffusion of plasma membrane proteins has been studied using the fluorescence recovery after photobleaching (FRAP) technique in cells under normal conditions. The following plot has been obtained under different conditions (e.g., when the signaling pathway is turned on). The protein binds to a few other proteins and protein complexes in the plasma membrane, resulting in a reduced lateral diffusion coefficient. Additionally, a significant fraction of the molecules of this prot
numerade.com
Jul 19, 2022
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